| Newsletter No. 441
|| April 19, 2021
| METHODS - Evolving data standards for cryo-EM structures
The development of cryo-EM is directly reflected by the growth of cryo-EM strructure depositions contributed worldwide to public data archived. The archiving systems and underlying data standards supporting depostioin, annotation, release, and validation of cyro-EM stuctures and the associated metadata describing cryo-EM experiments have been developed over time to support this growth. Recently, CL Lawson, HM Berman, and W Chiu outlined the history of these systems and described the process by which data standards had been developed. For details, please click the link.
Reliable cryo-EM resolution estimation
A modified Fourier shell correlation (FSC) moehodology is introduced by Pawel Penczek, which is aimed addressing two fundamental problems that mar the use of FSC: the strong influence of mask-induced artifacts on resolution estimation and the lack of assessment of FSC uncertaninties stemming from the inability to determine the asscociated number of degrees of freedom. For details, please click the link.
New approach for structure determination from tiny crystals
Many crystals can't be grown into crystals large enough for their atomic arrangements to be deciphered. To tackle this challenge, scientists at the U.S. DOE's Brookhaven National Laboratory and colleagues at Columbia University have developed a new approach for solving protein structure from tiny crystal. For details, please click the link.
ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation, Biophysical Methods, Sample Preparation.
|DISCUSSIONS - Suggestions?
ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, NCS, Missing Atoms, Trends in Crystallography, Absorption Correction, Data for Refinement and Publication, Validation, Table 1.
| READINGS AND TUTORIALS - Joachim Frank, Single-Partical Cryo-EM: Visualizing Biological Molecules in Their Native State
DR. ZBIGNIEW DAUTER'S LECTURES AT THE NIH (2005)
Part 1: "How to read international tables?"
Part 2: "Data collection strategy" and "Twinning"
"Phasing methods - a general introduction to all methods"
Part 3: "SAD phasing, Quick halide soaking, and Radiation damage
with possible use of it for phasing"
LOW RESOLUTION PHASING AND REFINEMENT (2011)
Data Collection Strategies (2013)
STRUCTURE FACTOR TUTORIAL (2014)
DATA COLLECTION FOR STRUCTURE DETERMENATION (2014)
CRYSTAL CLEAR (2014)
LITERATURE ON CRYSTALLOGRAPHY THEORY AND METHODS (2017)
PROTEIN CRYSTALLOGRAPHY Methods and Protocols (2017)
Understanding Biology Through Structure (2017)
On the state of crystallography at the dawn of the electron microscopy revolution (2017)
The Fascinating World of Crystallography (2018)
Data science skills for referees: I biological X-ray crystallography (2018)
Correcting the public record of biological crystallography (2019)
A Little Dictionary of Crystallography (2019)
Early history of cryo-cooling for macromolecular crystallography (2019)
Methods Comparison: XFEL vs microED (2020)
The data universe of structural biology (2020)
MicroED Methodology & Development (2020)
How to Speak, Lecture by Patrick Winston (2020)
Nobel Lecture of Jennifer Doudna (2020)
| LINKS - PDB SARS-CoV-2 Resources (Link)
Databases: DisProt, ExPASy, HAD, HIC-Up, NDB, PDB, PDBe, Protein Geometry
SARS-CoV-2 Related Structures, PDB SARS-CoV-2 Resources,
Programs: CCP4, COOT, DSSR, HKL, PHENIX, PyMOL, SOLVE, XDS
Servers: ACHESYM, Anisotropy, C6@C3, Dali, ESPript, Grade, PDBePISA, Phyre2,
|Copyright © NIH X-Ray Diffraction Group Maintained by Dr. Xinhua Ji|