pKM631

Description:	This vector produces an MBP-NusG-His6 fusion protein with a canonical TEV protease recognition site (ENLYFQG) between MBP and NusG-His6.
Origin:	A derivative of pMal-C2 (New England Biolabs)
Replicon:	ColE1 (pBR322)
Antibotic Resistance:	Ampicillin
Promotor:	tac
Inducer:	IPTG (1 mM)
Notes:	The MBP-NusG-His6 fusion protein does not bind well to amylose resin. It should be purified by IMAC (Ni-NTA) and gel filtration instead.
Literature Citations:	Kapust , R. B., Tozser, J., Copeland, T. D., and Waugh, D. S. (2002). The P1' specificity of tobacco etch virus protease. Biochem. Biophys. Res. Commun. 294: 949-955.
Nucleotide Sequence:

Amino Acid Sequence:

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTNSI TSLYKKAGSe nlyfqgMSEQ QVQELEKKWY ALQVEPGKEN EAKENLLKVL ELEGLKDLVD EVIVPAEEKV VIRAQGKEKY RLSLKGNARD ISVLGKKGVT TFRIENGEVK VVESVEGDTC VNAPPISKPG QKITCKENKT EAKIVLDNKI FPGYILIKAH MNDKLLMAIE KTPHVFRPVM VGGKPVPLKE EEVQNILNQI KRGVKPSKVE FEKGDQVRVI EGPFMNFTGT VEEVHPEKRK LTVMISIFGR MTPVELDFDQ VEKIHHHHHH