pKM596

Description:	A Gateway™ destination vector for the production of recombinant proteins as fusions to the C-terminus of Escherichia coli maltose-binding protein.
Origin:	A derivative of pMal-C2 (New England Biolabs)
Replicon:	ColE1 (pBR322)
Antibotic Resistance:	Ampicillin
Promotor:	tac
Inducer:	IPTG (1 mM)
Notes:	Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
Literature Citations:	Fox, J. D. and Waugh, D. S. (2003). Maltose-binding protein as a solubility enhancer. Methods. Mol. Biol. 205: 99-117.
Nucleotide Sequence:

Amino Acid Sequence: *

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTNSI TSLYKKAG...passenger protein. * Amino acid residues encoded by the attR1 site in the Gateway™ cloning cassette are underlined.