| Description: |
A Gateway™ destination
vector for the production of recombinant proteins as fusions to the C-terminus
of Vibrio cholerae maltose-binding protein. |
| |
|
| Origin: |
A derivative of pET11c
(Novagen) |
| |
|
| Replicon: |
ColE1 |
| |
|
| Antibotic
Resistance: |
Ampicillin |
| |
|
| Promotor: |
bacteriophage
T7 |
| |
|
| Inducer: |
IPTG (1 mM) |
| |
|
| Notes: |
Because it carries a gene
(ccdB ) that encodes a lethal DNA gyrase poison, this vector must be
propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen),
which is immune to the action of CcdB. |
| |
|
| Literature
Citations: |
Fox, J. D., Routzahn, K. M.,
Bucher, M. H., and Waugh, D. S. (2003). Maltodextrin-binding proteins from
diverse bacteria and archaea are potent solubility enhancers. FEBS Lett.
537: 53-57. |
| |
|
| Nucleotide
Sequence: |
 |
| |
|
