| Description: |
This baculovirus transfer
vector enables a protein of interest to be produced with a biotin acceptor
peptide (BAP) fused to its C-terminus in Spodoptera frugiperda cells.
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| Origin: |
A derivative of pFastBac-1
(Invitrogen) |
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| Replicon: |
ColE1/pUC (High copy
number) |
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| Antibotic
Resistance: |
Ampicillin,
Gentamycin |
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| Promotor: |
p10 (late) |
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| Inducer: |
Not
Applicable |
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| Notes: |
pDW465 also overproduces
E. coli biotin holoenzyme synthetase (BirA), under polyhedron promoter
control, so that BAP-tagged proteins can be biotinylated in Spodoptera
frugiperda cells. pDW464 is designed to use in conjunction with the
Bac-to-Bac system (Invitrogen) for constructing recombinant
baculoviruses. |
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| Literature
Citations: |
Duffy, S., Tsao, K.-L., and
Waugh, D. S. (1998). Efficient, site-specific biotinylation of recombinant
proteins in Spodoptera frugiperda cells using biotin acceptor
peptides. Anal. Biochem. 262: 122-128; Schatz. P. J. (1993). Use of peptide
libraries to map the substrate specificity of a peptide-modifying enzyme: a 13
residue consensus peptide specifies biotinylation in Escherichia coli.
Bio/Technology 11:1138-43. |
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| Nucleotide
Sequence: |
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