| Description: |
A Gateway™ destination
vector for the production of recombinant proteins as fusions to the C-terminus
of a dual His6-MBP tag in the periplasm of E. coli. |
| |
|
| Origin: |
A derivative of pMal-C2 (New
England Biolabs) |
| |
|
| Replicon: |
ColE1
(pBR322) |
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|
| Antibotic
Resistance: |
Ampicillin |
| |
|
| Promotor: |
tac |
| |
|
| Inducer: |
IPTG (1 mM) |
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|
| Notes: |
Because it carries a gene
(ccdB ) that encodes a lethal DNA gyrase poison, this vector must be
propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen),
which is immune to the action of CcdB. |
| |
|
| Literature
Citations: |
Nallamsetty, S., Austin, B.
P., Penrose, K. J., and Waugh, D. S. (2005). Gateway vectors for the production
of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and
periplasm of Escherichia coli. Protein Sci. 14:
2964-2971. |
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| Nucleotide
Sequence: |
 |
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