pDEST-periHisMBP

Description:	A Gateway™ destination vector for the production of recombinant proteins as fusions to the C-terminus of a dual His6-MBP tag in the periplasm of E. coli.
Origin:	A derivative of pMal-C2 (New England Biolabs)
Replicon:	ColE1 (pBR322)
Antibotic Resistance:	Ampicillin
Promotor:	tac
Inducer:	IPTG (1 mM)
Notes:	Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
Literature Citations:	Nallamsetty, S., Austin, B. P., Penrose, K. J., and Waugh, D. S. (2005). Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Protein Sci. 14: 2964-2971.
Nucleotide Sequence:

Amino Acid Sequence: *

MKIKTGARIL ALSALTTMMF SASALAKIHH HHHHEEGKLV IWINGDKGYN GLAEVGKKFE KDTGIKVTVE HPDKLEEKFP QVAATGDGPD IIFWAHDRFG GYAQSGLLAE ITPDKAFQDK LYPFTWDAVR YNGKLIAYPI AVEALSLIYN KDLLPNPPKT WEEIPALDKE LKAKGKSALM FNLQEPYFTW PLIAADGGYA FKYENGKYDI KDVGVDNAGA KAGLTFLVDL IKNKHMNADT DYSIAEAAFN KGETAMTING PWAWSNIDTS KVNYGVTVLP TFKGQPSKPF VGVLSAGINA ASPNKELAKE FLENYLLTDE GLEAVNKDKP LGAVALKSYE EELAKDPRIA ATMENAQKGE IMPNIPQMSA FWYAVRTAVI NAASGRQTVD EALKDAQTNS ITSLYKKAG….passenger protein. * Amino acid residues encoded by the attR1 site in the Gateway™ cloning cassette are underlined.