Newsletter 93
April 11, 2005


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Item 1: Meetings and Seminars

 
1. The 35th Mid-Atlantic Crystallography Meeting <http://www.niaid.nih.gov/dir/labs/lig/crystal/default.asp> May 11-13.

2. The NIH meeting "Structural Analysis of Large Macromolecular Assemblies: Sizing Up the Challenges" <http://pub.nigms.nih.gov/generic_meeting/index.cfm?id=7> June 2-3.

 

Item 2: March 2005 Publications by Members:

 

1:  Williams DC Jr, Cai M, Suh JY, Peterkofsky A, Clore GM.
Solution NMR structure of the 48 kDa IIAmannose-HPr complex of the Escherichia coli mannose phosphotransferase system.
J Biol Chem. 2005 Mar 23; [Epub ahead of print] PMID: 15788390

2:  Su HP, Garman SC, Allison TJ, Fogg C, Moss B, Garboczi DN.
The 1.51-A structure of the poxvirus L1 protein, a target of potent neutralizing antibodies.
Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4240-5. PMID: 15761054

3:  Mayer ML.
Some assembly required.
Nat Struct Mol Biol. 2005 Mar;12(3):208-9. PMID: 15744319

4:  Ramakrishnan B, Boeggeman E, Qasba PK.
Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I.
Biochemistry. 2005 Mar 8;44(9):3202-10. PMID: 15736931

5:  Caldovic L, Morizono H, Panglao MG, Lopez GY, Shi D, Summar ML, Tuchman M.
Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles.
Hum Mutat. 2005 Mar;25(3):293-8. PMID: 15714518

6:  Schubot FD, Jackson MW, Penrose KJ, Cherry S, Tropea JE, Plano GV, Waugh DS.
Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis.
J Mol Biol. 2005 Mar 4;346(4):1147-61. PMID: 15701523

7:  Tang C, Williams DC Jr, Ghirlando R, Clore GM.
Solution structure of enzyme IIA(Chitobiose) from the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.
J Biol Chem. 2005 Mar 25;280(12):11770-80. PMID: 15654077

8:  Gong X, Yu L, Xia D, Yu CA.
Evidence for electron equilibrium between the two hemes bL in the dimeric cytochrome bc1 complex.
J Biol Chem. 2005 Mar 11;280(10):9251-7. PMID: 15615714


Item 3: Tips and Tricks

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 4: Topic Discussion

HKL2000 has a built-in feature (Reprocess) to reintegrate raw date (frames) with unit cell parameters fixed at the values obtained after postrefinement using Scalepack. Any comments, experience, or recommendations?

Click for previous discussions on: Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 


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