Newsletter 85
December 20, 2004


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Item 1: November 2004 Publications by Members:

 

1:  Guan R, Roychowdhury A, Ember B, Kumar S, Boons GJ, Mariuzza RA.
Structural basis for peptidoglycan binding by peptidoglycan recognition proteins.
Proc Natl Acad Sci U S A. 2004 Nov 30; PMID: 15572450

2:  Swales K, Kakizaki S, Yamamoto Y, Inoue K, Kobayashi K, Negishi M.
Novel CAR-mediated mechanism for synergistic activation of two distinct elements within the human cytochrome P450 2B6 gene in HepG2 cells.
J Biol Chem. 2004 Nov 24; PMID: 15563456

3:  Derewenda U, Oleksy A, Stevenson AS, Korczynska J, Dauter Z, Somlyo AP, Otlewski J, Somlyo AV, Derewenda ZS.
The Crystal Structure of RhoA in Complex with the DH/PH Fragment of PDZRhoGEF, an Activator of the Ca(2+) Sensitization Pathway in Smooth Muscle.
Structure (Camb). 2004 Nov;12(11):1955-65. PMID: 15530360

4:  Forrer P, Chang C, Ott D, Wlodawer A, Pluckthun A.
Kinetic stability and crystal structure of the viral capsid protein SHP.
J Mol Biol. 2004 Nov 12;344(1):179-93. PMID: 15504410

5:  Saxena AK, Singh K, Long CA, Garboczi DN.
Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment.
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2054-7. Epub 2004 Oct 20. PMID: 15502325

6:  Schubot FD, Waugh DS.
A pivotal role for reductive methylation in the de novo crystallization of a ternary complex composed of Yersinia pestis virulence factors YopN, SycN and YscB.
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1981-6. Epub 2004 Oct 20. PMID: 15502305

7:  Zwart PH, Banumathi S, Dauter M, Dauter Z.
Radiation-damage-induced phasing with anomalous scattering: substructure solution and phasing.
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1958-63. Epub 2004 Oct 20. PMID: 15502302

8:  Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland TD, Waugh DS.
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro.
Protein Expr Purif. 2004 Nov;38(1):108-15. PMID: 15477088

9:  Squires EJ, Sueyoshi T, Negishi M.
Cytoplasmic Localization of Pregnane X Receptor and Ligand-dependent Nuclear Translocation in Mouse Liver.
J Biol Chem. 2004 Nov 19;279(47):49307-14. PMID: 15347657

10:  Vidal AE, Kannouche P, Podust VN, Yang W, Lehmann AR, Woodgate R.
Proliferating cell nuclear antigen-dependent coordination of the biological functions of human DNA polymerase iota.
J Biol Chem. 2004 Nov 12;279(46):48360-8. PMID: 15342632

 

11: Gu Y, Guo J, Pal A, Pan S-S, Zimniak P, Singh SV, and Ji X.

Crystal Structure of Human Glutathione S-Transferase A3-3 and Mechanistic Implications for Its High Steroid Isomerase Activity
Biochemistry. Release Date: 17-Nov-2004. DOI: 10.1021/bi048757g


Item 2: Tips and Tricks in Crystallography

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 3: Topic Discussion - Protein Expression and High-throughput Expression Systems

Click for previous discussions on: Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 


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