logo2a.gif
Newsletter No. 241
 July 4, 2011


Photo Gallery: The 41th Mid-Atlantic Crystallography Workshop





The XXII Congress and General Assembly of the IUCr (8/22-30/2011)

JUNE 2011 PUBLICATIONS BY MEMBERS OF THE GROUP  

1: Fairman JW, Noinaj N, Buchanan SK. The structural biology of β-barrel membrane
proteins: a summary of recent reports. Curr Opin Struct Biol. 2011 Jun 28. [Epub 
ahead of print] PubMed PMID: 21719274.

2: Kim SE, Bahta M, Lountos GT, Ulrich RG, Burke TR Jr, Waugh DS.
Isothiazolidinone (IZD) as a phosphoryl mimetic in inhibitors of the Yersinia
pestis protein tyrosine phosphatase YopH. Acta Crystallogr D Biol Crystallogr.
2011 Jul;67(Pt 7):639-45. Epub 2011 Jun 11. PubMed PMID: 21697602.

3: Sastry M, Xu L, Georgiev IS, Bewley CA, Nabel GJ, Kwong PD. Mammalian
production of an isotopically enriched outer domain of the HIV-1 gp120
glycoprotein for NMR spectroscopy. J Biomol NMR. 2011 Jun 12. [Epub ahead of
print] PubMed PMID: 21667299.

4: Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X.
Structural basis for RNA recognition by NusB and NusE in the initiation of
transcription antitermination. Nucleic Acids Res. 2011 Jun 7. [Epub ahead of
print] PubMed PMID: 21652641.

5: Tu C, Zhou X, Tarasov SG, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X. The 
Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end 
of 16S rRNA. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10156-61. Epub 2011
Jun 6. PubMed PMID: 21646538.

6: Engel A, Shewmaker F, Edskes HK, Dyda F, Wickner RB. Amyloid of the Candida
albicans Ure2p Prion Domain Is Infectious and Has an In-Register Parallel β-Sheet
Structure. Biochemistry. 2011 Jun 15. PubMed PMID: 21634787.

7: Boura E, Rózycki B, Herrick DZ, Chung HS, Vecer J, Eaton WA, Cafiso DS, Hummer
G, Hurley JH. Solution structure of the ESCRT-I complex by small-angle X-ray
scattering, EPR, and FRET spectroscopy. Proc Natl Acad Sci U S A. 2011 Jun
7;108(23):9437-42. Epub 2011 May 19. PubMed PMID: 21596998.

8: McLellan JS, Correia BE, Chen M, Yang Y, Graham BS, Schief WR, Kwong PD.
Design and characterization of epitope-scaffold immunogens that present the
motavizumab epitope from respiratory syncytial virus. J Mol Biol. 2011 Jun
24;409(5):853-66. Epub 2011 Apr 27. PubMed PMID: 21549714.

9: Hurley JH, Stenmark H. Molecular mechanisms of ubiquitin-dependent membrane
traffic. Annu Rev Biophys. 2011 Jun 9;40:119-42. PubMed PMID: 21332354.
TIPS AND TRICKS - Cryoprotectant

Jim Pflugraph (Rigaku): I always try sugars for everything.  There is a cryocrystallography webinar at rigaku.com with embedded videos on how to do this.  50% to 100% saturated sugar (sucrose, glucose, trehalose, sorbitol, et al.) in reservoir buffer is usually what I try.

                   

ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation.

TOPIC DISCUSSION - Low Resolution Crystallography (Click for PDF reader to view articles)


ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography,  Absorption Correction.
DR. ZBIGNIEW DAUTER'S LECTURE AT THE NIH (2005)
 

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

            with possible use of it for phasing"
 LINKS - New Addition: C6

Jason Stagno (NCI): C6 is a powerful tool that allows you to assess the similarity between commercial, C3 and custom designed screens.

Databases: BMCD, CryoD, DisProt, HAD, HIC-Up, NDB, PDB, PDBe, Swiss-Prot
Programs: CCP4, COOT, HKL, PHENIX, PyMOL, SOLVE, USF, XDS
Servers: Crystal, C6, PHYRE, Dali, ESPript, Phyre, Probity, Protein
 
Copyright © NIH X-Ray Diffraction Group                       Maintained by Dr. Xinhua Ji
on the NIH-NCI-CCR-MCL server (http://mcl1.ncifcrf.gov)