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Newsletter No. 232
January 11, 2011


The 55th Annual Meeting of Biophysical Society (3/5-11/2011)



The 2011 Meeting of the American Crystallographic Association (5/28-6/2/2011)




The XXII Congress and General Assembly of the IUCr (8/22-30/2011)


DECEMBER 2010 PUBLICATIONS BY MEMBERS OF THE GROUP  

1: Cai M, Huang Y, Craigie R, Clore GM. Structural Basis of the Association of
HIV-1 Matrix Protein with DNA. PLoS One. 2010 Dec 23;5(12):e15675. PubMed PMID:
21203471
; PubMed Central PMCID: PMC3009736.

2: Changela A, Wu X, Yang Y, Zhang B, Zhu J, Nardone GA, O'Dell S, Pancera M,
Gorny MK, Phogat S, Robinson JE, Stamatatos L, Zolla-Pazner S, Mascola JR, Kwong
PD
. Crystal structure of human antibody 2909 reveals conserved features of
quaternary-specific antibodies that potently neutralize HIV-1. J Virol. 2010 Dec
29. [Epub ahead of print] PubMed PMID: 21191009.

3:
Clore GM. Exploring sparsely-populated states of macromolecules by diamagnetic
and paramagnetic NMR relaxation. Protein Sci. 2010 Dec 17. [Epub ahead of print]
PubMed PMID: 21171048.

4: Takayama Y, Schwieters CD, Grishaev A, Ghirlando R,
Clore GM. Combined Use of
Residual Dipolar Couplings and Solution X-ray Scattering To Rapidly Probe
Rigid-Body Conformational Transitions in a Non-phosphorylatable Active-Site
Mutant of the 128 kDa Enzyme I Dimer. J Am Chem Soc. 2010 Dec 16. [Epub ahead of
print] PubMed PMID: 21162528.

5: Grundy GJ, Yang W, Gellert M. Autoinhibition of DNA cleavage mediated by RAG1
and RAG2 is overcome by an epigenetic signal in V(D)J recombination. Proc Natl
Acad Sci U S A. 2010 Dec 28;107(52):22487-92. Epub 2010 Dec 13. PubMed PMID:
21149691
.

6:
Hurley JH, Boura E, Carlson LA, Różycki B. Membrane budding. Cell. 2010 Dec
10;143(6):875-87. PubMed PMID: 21145455.

7: Horton JK, Stefanick DF, Zeng JY, Carrozza MJ, Wilson SH. Requirement for NBS1
in the S phase checkpoint response to DNA methylation combined with PARP
inhibition. DNA Repair (Amst). 2010 Dec 2. [Epub ahead of print] PubMed PMID:
21130714
.

8: Li M, Gustchina A, Glesner J, Wünschmann S, Vailes LD, Chapman MD, Pomés A,
Wlodawer A. Carbohydrates contribute to the interactions between cockroach
allergen Bla g 2 and a monoclonal antibody. J Immunol. 2011 Jan 1;186(1):333-40.
Epub 2010 Dec 1. PubMed PMID: 21123808.

9:
Yang W. Topoisomerases and site-specific recombinases: similarities in
structure and mechanism. Crit Rev Biochem Mol Biol. 2010 Dec;45(6):520-34. PubMed
PMID: 21087076
.

TIPS AND TRICKS - <I/sigmaI> and <I>/<sigmaI>

Ed Pozharski's python script at the ccp4wiki reads a .sca file and produces a table with both <I/sigmaI> and <I>/<sigmaI> for a user-defined number of shells.

ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation.

TOPIC DISCUSSION - Resolution (Click for PDF reader to view articles)

Super-resolution biomolecular crystallography with low-resolution data

Is too 'creative' language acceptable in crystallography?

Review: Resolution cutoff criteria

Discussion: Super-resolution Part 1,
Part 2, Part 3, Part 4, Part 5

ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, Structural Genomics, NCS, Missing Atoms, Trends in CrystallographyAbsorption Correction.

DR. ZBIGNIEW DAUTER'S LECTURE AT THE NIH (2005)
 

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

            with possible use of it for phasing"


 LINKS - Recommended Sites?

Databases: BMCD, CryoD, HAD, HIC-Up, NDBPDB, PDBe, Swiss-Prot
Programs: CCP4, COOT, HKLPHENIX, PyMOL, SOLVE, USF, XDS
Servers: Crystal, PHYRE, Dali, ESPript, Probity, Protein
 
Copyright © NIH X-Ray Diffraction Group                       Maintained by Dr. Xinhua Ji
on the NIH-NCI-CCR-MCL server (http://mcl1.ncifcrf.gov)