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Newsletter No. 207
 November 2, 2009

The Nobel Prize in Chemistry 2009

"for the studies of the structure and function of the ribosome"

Congratulations!

OCTOBER 2009 PUBLICATIONS BY MEMBERS OF THE GROUP  

1: Kwong PD, Mascola JR, Nabel GJ. Mining the B cell repertoire for broadly
neutralizing monoclonal antibodies to HIV-1. Cell Host Microbe. 2009;6:292-4. 
PubMed: 19837366.

2: Dauter Z, Wlodawer A. Proteins do not have strong spines after all. Structure.
2009;17:1278-9. PubMed: 19836327.

3: Gustchina E, Louis JM, Frisch C, Ylera F, Lechner A, Bewley CA, Clore GM.
Affinity maturation by targeted diversification of the CDR-H2 loop of a
monoclonal Fab derived from a synthetic naïve human antibody library and directed
against the internal trimeric coiled-coil of gp41 yields a set of Fabs with
improved HIV-1 neutralization potency and breadth. Virology. 2009;393:112-9. 
PubMed: 19695655.

4: Liu Y, Prasad R, Beard WA, Hou EW, Horton JK, McMurray CT, Wilson SH.
Coordination between polymerase beta and FEN1 can modulate CAG repeat expansion. 
J Biol Chem. 2009;284:28352-66. PubMed: 19674974.

5: Hu K, Doucleff M, Clore GM. Using multiple quantum coherence to increase the
15N resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and 
RDC measurements. J Magn Reson. 2009;200:173-7. PubMed: 19615926.
TIPS AND TRICKS

* Sean Seaver: Crystallization Resources - P212121

* Artem Evdokimov: Mounting needle-like crystals for data collection

* P. Hubbard: Crystal Screen Optimization Server

It is a GUI written by P Hubbard to calculate 2D gradients for crystal screen optimization (much like a web 2.0 version of Hampton’s “Make Tray” utility). It gives a handy print out of recipes and records for people who make their optimization trays by hand.

* Japan AEA: Cryoprotectant Database

* CCP4 wiki: Crystallization of Protein-DNA Complexes

* Annie Hassell: Hampton Research Crystal Screens 1 & 2 as Additives

Annie Hassell and co-workers at Glaxo Smithkline have had lots of success using the Hampton Research Crystal Screens (HRCS) 1 & 2 as additives. Sometimes these improve the crystal quality whereas the 96 Additive Screen does not. What they have not had a great deal of success with is determining which component of the added reagent is responsible for improving the crystal quality. Does not appear that it is just one component in many cases.

They generally use 5% of the sparse matrix screen added to the well. When they get a hit, they further optimize the concentration of the reagent to add to the well.

ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation.

TOPIC DISCUSSION - Suggested Topics?

ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography,  Absorption Correction.
DR. ZBIGNIEW DAUTER'S LECTURE AT THE NIH (2005)
 

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

            with possible use of it for phasing"
Copyright © NIH X-Ray Diffraction Group                       Maintained by Dr. Xinhua Ji
on the NIH-NCI-CCR-MCL server (http://mcl1.ncifcrf.gov)