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AUGUST 2009 PUBLICATIONS BY MEMBERS
OF
THE GROUP
1: Brzuszkiewicz A, Nowak E, Dauter Z, Dauter M, Cieśliński H, Długołecka A, Kur
J. Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A
covalently inhibited by monoethylphosphonate. Acta Crystallogr Sect F Struct Biol
Cryst Commun. 2009 Sep 1;65(Pt 9):862-5. PubMed PMID: 19724118.
2: Carrozza MJ, Stefanick DF, Horton JK, Kedar PS, Wilson SH. PARP inhibition
during alkylation-induced genotoxic stress signals a cell cycle checkpoint
response mediated by ATM. DNA Repair (Amst). 2009 Aug 28. PubMed PMID: 19717351.
3: Tu C, Zhou X, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X. Structure of ERA
in complex with the 3' end of 16S rRNA: Implications for ribosome biogenesis.
Proc Natl Acad Sci U S A. 2009 Aug 17. PubMed PMID: 19706445.
4: Gustchina E, Louis JM, Frisch C, Ylera F, Lechner A, Bewley CA, Clore GM.
Affinity maturation by targeted diversification of the CDR-H2 loop of a
monoclonal Fab derived from a synthetic naïve human antibody library and directed
against the internal trimeric coiled-coil of gp41 yields a set of Fabs with
improved HIV-1 neutralization potency and breadth. Virology. 2009 Aug 18.
PubMed PMID: 19695655.
5: Wu X, Zhou T, O'Dell S, Wyatt RT, Kwong PD, Mascola JR. Mechanism of HIV-1
Resistance to Monoclonal Antibody b12 that Effectively Targets the Site of CD4
Attachment. J Virol. 2009 Aug 19. PubMed PMID: 19692465.
6: Nowak E, Brzuszkiewicz A, Dauter M, Dauter Z, Rosenbaum G. To scavenge or not
to scavenge: that is the question. Acta Crystallogr D Biol Crystallogr. 2009
Sep;65(Pt 9):1004-6. Epub 2009 Aug 14. PubMed PMID: 19690379.
7: Pletnev S, Morozova KS, Verkhusha VV, Dauter Z. Rotational order-disorder
structure of fluorescent protein FP480. Acta Crystallogr D Biol Crystallogr. 2009
Sep;65(Pt 9):906-12. Epub 2009 Aug 6. PubMed PMID: 19690368.
8: Im YJ, Wollert T, Boura E, Hurley JH. Structure and function of the
ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell. 2009
Aug;17(2):234-43. PubMed PMID: 19686684.
9: Dorjsuren D, Wilson DM 3rd, Beard WA, McDonald JP, Austin CP, Woodgate R,
Wilson SH, Simeonov A. A real-time fluorescence method for enzymatic
characterization of specialized human DNA polymerases. Nucleic Acids Res. 2009
Aug 14. PubMed PMID: 19684079.
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TIPS AND TRICKS
(1) Cryoprotectant
Database
(2) CCP4
wiki on Crystallization of Protein-DNA Complexes
(3) Hampton Research Crystal Screens 1
& 2 as Additives
Annie Hassell
and co-workers at Glaxo Smithkline have had lots of success using the
Hampton Research Crystal Screens (HRCS) 1 & 2 as additives.
Sometimes these improve the crystal quality whereas the 96 Additive
Screen does not. What they have not had a great deal of success with is
determining which component of the added reagent is responsible for
improving the crystal quality. Does not appear that it is just one
component in many cases.
They
generally use 5% of the sparse matrix screen added to the well. When
they get a hit, they further optimize the concentration of the
reagent to add to the well.
ARCHIVE: Introduction, Pre-crystallization,
Crystallization, Post-crystallization,
Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution,
Structure
Refinement, Structure
Analysis & Presentation.
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