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Newsletter No. 202
 August 24, 2009

2009 Meeting of American Crystallographic Association (07/25-30)

JULY 2009 PUBLICATIONS BY MEMBERS OF THE GROUP  

1: Grundy GJ, Ramón-Maiques S, Dimitriadis EK, Kotova S, Biertümpfel C, Heymann
JB, Steven AC, Gellert M, Yang W. Initial stages of V(D)J recombination: the
organization of RAG1/2 and RSS DNA in the postcleavage complex. Mol Cell. 2009
Jul 31;35(2):217-27. PMID: 19647518.
   
2: Wang R, Natarajan K, Margulies DH. Structural basis of the CD8alphabeta/MHC
class i interaction: focused recognition orients CD8beta to a T cell proximal
position. J Immunol. 2009 Aug 15;183(4):2554-64. PMID: 19625641.
   
3: Botos I, Liu L, Wang Y, Segal DM, Davies DR. The Toll-like receptor 3:dsRNA
signaling complex. Biochim Biophys Acta. 2009 Jul 9. PMID: 19595807.
   
4: Ryabov Y, Suh JY, Grishaev A, Clore GM, Schwieters CD. Using the
experimentally determined components of the overall rotational diffusion tensor
to restrain molecular shape and size in NMR structure determination of globular
proteins and protein-protein complexes. J Am Chem Soc. 2009 Jul
15;131(27):9522-31. PMID: 19537713.
   
5: Wollert T, Yang D, Ren X, Lee HH, Im YJ, Hurley JH. The ESCRT machinery at a
glance. J Cell Sci. 2009 Jul 1;122(Pt 13):2163-6. PMID: 19535731.
TIPS AND TRICKS

(1) Cryoprotectant Database

(2) CCP4 wiki on Crystallization of Protein-DNA Complexes

(3) Hampton Research Crystal Screens 1 & 2 as Additives

Annie Hassell and co-workers at Glaxo Smithkline have had lots of success using the Hampton Research Crystal Screens (HRCS) 1 & 2 as additives. Sometimes these improve the crystal quality whereas the 96 Additive Screen does not. What they have not had a great deal of success with is determining which component of the added reagent is responsible for improving the crystal quality. Does not appear that it is just one component in many cases.

They generally use 5% of the sparse matrix screen added to the well. When they get a hit, they further optimize the concentration of the reagent to add to the well.

ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation.

TOPIC DISCUSSION - Suggested Topics?

ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography,  Absorption Correction.
DR. ZBIGNIEW DAUTER'S LECTURE AT THE NIH (2005)
 

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

            with possible use of it for phasing"
Copyright © NIH X-Ray Diffraction Group                       Maintained by Dr. Xinhua Ji
on the NIH-NCI-CCR-MCL server (http://mcl1.ncifcrf.gov)