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JUNE 2009 PUBLICATIONS BY MEMBERS OF
THE GROUP
1: Magracheva E, Kozlov S, Stewart CL, Wlodawer A, Zdanov A. Structure of the lamin A/C R482W mutant responsible for dominant familial partial lipodystrophy (FPLD). Acta Crystallogr Sect F 65:665-70. PMID: 19574635. 2: Jiang L, Duriseti S, Sun P, Miller LH. Molecular basis of binding of the Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C. Mol Biochem Parasitol. 2009 Jun 26. PMID: 19563830. 3: Das R, Mariano J, Tsai YC, Kalathur RC, Kostova Z, Li J, Tarasov SG, McFeeters RL, Altieri AS, Ji X, Byrd RA, Weissman AM. Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78. Mol Cell. 34:674-85. PMID: 19560420. 4: Clore GM, Iwahara J. Theory, Practice, and Applications of Paramagnetic Relaxation Enhancement for the Characterization of Transient Low-Population States of Biological Macromolecules and Their Complexes. Chem Rev. 2009 Jun 12. PMID: 19522502. 5: Miller M. The importance of being flexible: the case of basic region leucine zipper transcriptional regulators. Curr Protein Pept Sci. 10:244-69. PMID: 19519454. 6: Li YF, Poole S, Nishio K, Jang K, Rasulova F, McVeigh A, Savarino SJ, Xia D, Bullitt E. Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli. Proc Natl Acad Sci U S A. 106:10793-8. PMID: 19515814. 7: Ramakrishnan B, Boeggeman E, Manzoni M, Zhu Z, Loomis K, Puri A, Dimitrov DS, Qasba PK. Multiple Site-Specific in Vitro Labeling of Single-Chain Antibody. Bioconjug Chem. 2009 Jun 9. PMID: 19507852. 8: Kwong PD, Wilson IA. HIV-1 and influenza antibodies: seeing antigens in new ways. Nat Immunol. 10:573-8. Review. PMID: 19448659. 9: Boeggeman E, Ramakrishnan B, Pasek M, Manzoni M, Puri A, Loomis KH, Waybright TJ, Qasba PK. Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection. Bioconjug Chem. 20:1228-36. PMID: 19425533.
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| TIPS AND TRICKS - Hampton Research Crystal Screens 1
& 2 as Additives
Annie Hassell
and co-workers at Glaxo Smithkline have had lots of success using the
Hampton Research Crystal Screens (HRCS) 1 & 2 as additives.
Sometimes these improve the crystal quality whereas the 96 Additive
Screen does not. What they have not had a great deal of success with is
determining which component of the added reagent is responsible for
improving the crystal quality. Does not appear that it is just one
component in many cases.
They
generally use 5% of the sparse matrix screen added to the well. When
they get a hit, they further optimize the concentration of the
reagent to add to the well.
ARCHIVE: Introduction, Pre-crystallization,
Crystallization, Post-crystallization,
Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution,
Structure
Refinement, Structure
Analysis & Presentation.
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DR. ZBIGNIEW DAUTER'S LECTURE AT
THE NIH (2005)
Part 1: "How to read
international tables?"
Part 2: "Data
collection strategy" and "Twinning"
"Phasing methods - a general introduction to all methods"
Part 3: "SAD
phasing, Quick halide soaking, and Radiation damage
with possible use of it for phasing"
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