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Newsletter No. 200
July 27, 2009

2009 Meeting of American Crystallographic Association (07/25-30)

JUNE 2009 PUBLICATIONS BY MEMBERS OF THE GROUP  

1: Magracheva E, Kozlov S, Stewart CL, Wlodawer A, Zdanov A. Structure of the
lamin A/C R482W mutant responsible for dominant familial partial lipodystrophy
(FPLD). Acta Crystallogr Sect F 65:665-70. PMID: 19574635.

2: Jiang L, Duriseti S, Sun P, Miller LH. Molecular basis of binding of the
Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C. Mol
Biochem Parasitol. 2009 Jun 26. PMID: 19563830.

3: Das R, Mariano J, Tsai YC, Kalathur RC, Kostova Z, Li J, Tarasov SG, McFeeters
RL, Altieri AS, Ji X, Byrd RA, Weissman AM. Allosteric activation of E2-RING
finger-mediated ubiquitylation by a structurally defined specific E2-binding
region of gp78. Mol Cell. 34:674-85. PMID: 19560420.

4: Clore GM, Iwahara J. Theory, Practice, and Applications of Paramagnetic
Relaxation Enhancement for the Characterization of Transient Low-Population
States of Biological Macromolecules and Their Complexes. Chem Rev. 2009 Jun 12.
PMID: 19522502.

5: Miller M. The importance of being flexible: the case of basic region leucine
zipper transcriptional regulators. Curr Protein Pept Sci. 10:244-69. PMID: 19519454.

6: Li YF, Poole S, Nishio K, Jang K, Rasulova F, McVeigh A, Savarino SJ,
Xia D,
Bullitt E. Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli.
Proc Natl Acad Sci U S A. 106:10793-8. PMID: 19515814.

7: Ramakrishnan B, Boeggeman E, Manzoni M, Zhu Z, Loomis K, Puri A, Dimitrov DS,
Qasba PK. Multiple Site-Specific in Vitro Labeling of Single-Chain Antibody.
Bioconjug Chem. 2009 Jun 9. PMID: 19507852.

8:
Kwong PD, Wilson IA. HIV-1 and influenza antibodies: seeing antigens in new
ways. Nat Immunol. 10:573-8. Review. PMID: 19448659.

9: Boeggeman E, Ramakrishnan B, Pasek M, Manzoni M, Puri A, Loomis KH, Waybright
TJ, Qasba PK. Site specific conjugation of fluoroprobes to the remodeled Fc
N-glycans of monoclonal antibodies using mutant glycosyltransferases: application
for cell surface antigen detection. Bioconjug Chem. 20:1228-36. PMID: 19425533.


TIPS AND TRICKS Hampton Research Crystal Screens 1 & 2 as Additives

Annie Hassell and co-workers at Glaxo Smithkline have had lots of success using the Hampton Research Crystal Screens (HRCS) 1 & 2 as additives. Sometimes these improve the crystal quality whereas the 96 Additive Screen does not. What they have not had a great deal of success with is determining which component of the added reagent is responsible for improving the crystal quality. Does not appear that it is just one component in many cases.

They generally use 5% of the sparse matrix screen added to the well. When they get a hit, they further optimize the concentration of the reagent to add to the well.

ARCHIVE: Introduction, Pre-crystallization, Crystallization, Post-crystallization, Derivatization, Cryoprotection, Diffraction, Symmetry, Structure Solution, Structure Refinement, Structure Analysis & Presentation.

TOPIC DISCUSSION - Suggested Topics?

ARCHIVE: Test-set-and-R-free, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Expression, Structural Genomics, NCS, Missing Atoms, Trends in CrystallographyAbsorption Correction.

DR. ZBIGNIEW DAUTER'S LECTURE AT THE NIH (2005)
 

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

            with possible use of it for phasing"

Copyright © NIH X-Ray Diffraction Group                       Maintained by Dr. Xinhua Ji
on the NIH-NCI-CCR-MCL server (http://mcl1.ncifcrf.gov)