Newsletter 187
Happy Lunar New Year 2009!
January 26, 2009

The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

2009 Mid-Atlantic Macromolecular Crystallography Meeting (05/28-30)

2009 Meeting of American Crystallographic Association (07/25-30)

Item 1: December 2008 Publications by Members of the Group

   
1: Sauna ZE, Bohn SS, Rutledge R, Dougherty MP, Cronin S, May L, Xia D, Ambudkar 
SV, Golin J.
 Mutations Define Cross-talk between the N-terminal Nucleotide-binding Domain and 
Transmembrane Helix-2 of the Yeast Multidrug Transporter Pdr5: POSSIBLE
CONSERVATION OF A SIGNALING INTERFACE FOR COUPLING ATP HYDROLYSIS TO DRUG
TRANSPORT.
J Biol Chem. 2008 Dec 12;283(50):35010-22. PMID: 18842589 
    
2: Davis J, Wang J, Tropea JE, Zhang D, Dauter Z, Waugh DS, Wlodawer A.
 Novel fold of VirA, a type III secretion system effector protein from Shigella
flexneri.
Protein Sci. 2008 Dec;17(12):2167-73. PMID: 18787201

Item 2: Tips and Tricks

Click for Introduction and tips and tricks in Pre-crystallization modification, Crystallization, Post-crystallization treatment, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis & Presentation.

Item 3: Topic Discussion - Validation

Click for previous discussions on: Validation, Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

"Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage

with possible use of it for phasing"

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).