Newsletter 168
March 10, 2008


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

38th Mid-Atlantic Macromolecular Crystallography Meeting
22 - 24 May 2008, Chapel Hill, NC, USA

2008 Meeting of the American Crystallography Association
31 May - 5 June 2008, Knoxville, TN, USA

21st Congress of the International Union of Crystallography 2008
23 - 31 August 2008, Osaka, Japan

 

Item 1: February 2008 Publications by Members of the Group

1: Inoue K, Negishi M.
Nuclear receptor CAR requires early growth response 1 to
activate the human cytochrome P450 2B6 gene.
J Biol Chem. 2008 Feb 25; PMID: 18303024

2: Lin DY, Tanaka Y, Iwasaki M, Gittis AG, Su HP, Mikami B,
Okazaki T, Honjo T, Minato N, Garboczi DN.
The PD-1/PD-L1 complex resembles the antigen-binding Fv
domains of antibodies and T cell receptors.
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3011-6.
PMID: 18287011

3: Hu J, Hu K, Williams DC Jr, Komlosh M, Cai M, Clore GM.
Solution nmr structures of productive and non-productive
complexes between the A and B domains of the cytoplasmic
subunit of the mannose transporter of the Escherichia coli
phosphotransferase system.
J Biol Chem. 2008 Feb 11; PMID: 18270202

4: Wickner RB, Dyda F, Tycko R.
Amyloid of Rnq1p, the basis of the [PIN+] prion, has a
parallel in-register beta-sheet structure.
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2403-8.
PMID: 18268327

5: Ramakrishnan B, Boeggeman E, Qasba PK.
Applications of glycosyltransferases in the site-specific
conjugation of biomolecules and the development of a
targeted drug delivery system and contrast agents for MRI.
Expert Opin Drug Deliv. 2008 Feb;5(2):149-153.
PMID: 18248315


Item 2: Tips and Tricks (Click for PDF reader to view article)

Need an image processing software that builds three-dimensional profiles? Try XDS. For an easy start, see INTEGRATE by Kay Diederichs.

Recommended viewing: How long will my crystal last?

Dr. Susan Buchanan (NIDDK): Crystallization of Integral Membrane Proteins
    X-ray crystallography has become a very powerful tool for determining the structures of integral membrane proteins, with almost 200 unique membrane protein structures solved as of December 2007 (for a complete list, see Stephen White’s summary at http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html). However, membrane protein structures still represent less than 1% of all structures in the PDB. The major bottlenecks in the field are the expression of sufficient quantities of functional membrane proteins and the growth of well ordered crystals for X-ray analysis. This short review covers only aspects pertaining to crystallization. Our approach to membrane protein crystallization was recently written up for Current Protocols in Protein Science (Unit 17.9, available from the NIH library website). An interesting review has been written by Patrick Loll1. (Full Article)

Click for Introduction and tips and tricks in Pre-crystallization modification, Crystallization, Post-crystallization treatment, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis & Presentation.


Item 3: Topic Discussion

Click for previous discussions on: Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"


This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).