March 10, 2008
The NIH X-Ray Diffraction Interest Group
web site: http://mcl1.ncifcrf.gov/nihxray
38th Mid-Atlantic Macromolecular Crystallography Meeting
2008 Meeting of the American Crystallography Association
Congress of the International Union of Crystallography
Item 1: February 2008 Publications by Members of the Group
1: Inoue K, Negishi M.
Item 2: Tips and Tricks (Click for PDF reader to view article)
Need an image processing software that builds three-dimensional profiles? Try XDS. For an easy start, see INTEGRATE by Kay Diederichs.
Recommended viewing: How long will my crystal last?
Dr. Susan Buchanan (NIDDK): Crystallization of Integral Membrane Proteins
X-ray crystallography has become a very powerful tool for determining the structures of integral membrane proteins, with almost 200 unique membrane protein structures solved as of December 2007 (for a complete list, see Stephen White’s summary at http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html). However, membrane protein structures still represent less than 1% of all structures in the PDB. The major bottlenecks in the field are the expression of sufficient quantities of functional membrane proteins and the growth of well ordered crystals for X-ray analysis. This short review covers only aspects pertaining to crystallization. Our approach to membrane protein crystallization was recently written up for Current Protocols in Protein Science (Unit 17.9, available from the NIH library website). An interesting review has been written by Patrick Loll1. (Full Article)
Click for Introduction and tips and tricks in Pre-crystallization modification, Crystallization, Post-crystallization treatment, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis & Presentation.
3: Topic Discussion
Click for previous discussions on: Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.
Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (2005)
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