January 14, 2008
The NIH X-Ray Diffraction Interest Group
web site: http://mcl1.ncifcrf.gov/nihxray
2008 Meeting of the American Crystallography Association
Congress of the International Union of Crystallography
Item 1: December 2007 Publications by Members of the Group
Item 2: Tips and Tricks
Recommended viewing: How long will my crystal last? (Click for PDF reader to view article)
Dr. Susan Buchanan (NIDDK): Crystallization of Integral Membrane Proteins
X-ray crystallography has become a very powerful tool for determining the structures of integral membrane proteins, with almost 200 unique membrane protein structures solved as of December 2007 (for a complete list, see Stephen White’s summary at http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html). However, membrane protein structures still represent less than 1% of all structures in the PDB. The major bottlenecks in the field are the expression of sufficient quantities of functional membrane proteins and the growth of well ordered crystals for X-ray analysis. This short review covers only aspects pertaining to crystallization. Our approach to membrane protein crystallization was recently written up for Current Protocols in Protein Science (Unit 17.9, available from the NIH library website). An interesting review has been written by Patrick Loll1. (Full Article) (Click for PDF reader to view article)
3: Topic Discussion
Click for previous discussions on: Twinning, Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.
Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (2005)
This site is maintained by Dr. Xinhua Ji (firstname.lastname@example.org) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).