Newsletter 154
August 13, 2007

The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

The 9th International Conference on Biology and Synchrotron Radiation
13-17 August 2007, Manchester, England

The 21st Congress of the Internatinal Union of Crystallography
23 - 31 August 2008, Osaka, Japan

Item 1: July 2007 Publications by Members of the Group

1: Gan J, Wu Y, Prabakaran P, Gu Y, Li Y, Andrykovitch M, Liu H, 
Gong Y, Yan H, Ji X.
 Structural and Biochemical Analyses of Shikimate Dehydrogenase 
AroE from Aquifex aeolicus: Implications for the Catalytic 
Mechanism.
Biochemistry. 2007 Jul 25; PMID: 17649975
   
2: Moon AF, Garcia-Diaz M, Batra VK, Beard WA, Bebenek K, 
Kunkel TA, Wilson SH, Pedersen LC.
 The X family portrait: Structural insights into biological 
functions of X family polymerases.
DNA Repair (Amst). 2007 Jul 11; PMID: 17631059 
   
3: Lin G, Bertolotti-Ciarlet A, Haggarty B, Romano J, McGeehan K, 
Leslie G, d'Oliviere AP, Huang CC, Kwong PD, Doms RW, Hoxie JA.
 Replication competent variants of human immunodeficiency virus 
type-2 lacking the V3 loop exhibit resistance to chemokine 
receptor antagonists.
J Virol. 2007 Jul 3; PMID: 17609282

Item 2: Tips and Tricks

Click for Introduction and tips and tricks in Pre-crystallization modification, Crystallization, Post-crystallization treatment, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

Item 3: Topic Discussion - Twinning

Please share your experience in twinning by presenting a case study. To learn or to review the basics of twinning, see Paul Adams' presentation.

Click for previous discussions on: Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

"Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage

with possible use of it for phasing"

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).