Newsletter 142
February 26, 2007


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

The 4th annual SER-CAT Symposium 16 March 2007, National Cancer Institute at Frederick, Frederick, MD, USA

The 2007 Meeting of the American Crystallographic Associstion 21-26 July 2007, Salt Lake City, UT, USA

The 9th International Conference on Biology and Synchrotron Radiation 13-17 August 2007, Manchester, England


Item 1: January 2007 Publications by Members:

1:  Guan R, Mariuzza RA. 
 Peptidoglycan recognition proteins of the innate immune system.
Trends Microbiol. 2007 Jan 31; PMID: 17275309 

2:  Chaudhuri R, Lindwasser OW, Smith WJ, Hurley JH, Bonifacino JS.
 CD4 downregulation by HIV-1 Nef is dependent on clathrin and 
involves a direct interaction of Nef with the AP2 clathrin adaptor.
J Virol. 2007 Jan 31; PMID: 17267500 

3:  Nakamura K, Moore R, Negishi M, Sueyoshi T. 
 Nuclear pregnane X receptor cross-talk with FOXA2 to mediate the 
drug-induced regulation of lipid metabolism in fasting mouse liver.
J Biol Chem. 2007 Jan 30; PMID: 17267396 

4:  Wally J, Buchanan SK. 
 A structural comparison of human serum transferrin and human 
lactoferrin.
Biometals. 2007 Jan 11; PMID: 17216400 

5:  Wlodawer A. 
 Natalia Sergeevna Andreeva 1922-2006.
Nat Struct Mol Biol. 2007 Jan;14(1):2. PMID: 17203064

Item 2: Tips and Tricks

Wei Yang (NIDDK): Crystallization of Protein-DNA Complexes (updated)
    Macromolecular interaction is essential, necessary and unavoidable in a living organism. Specific interactions among macromolecules are required for molecular machinery assembly and for progression and regulation of metabolic reactions.  To fully understand a biological process, it is essential to determine the atomic structures of and interactions among components of a macromolecular complex and to decipher how these structures and interactions change during a reaction or signaling cycle.  Some macromolecular complexes are naturally stable, for example tetrameric hemoglobin, nucleosome, and ribosome. But most macromolecular complexes are formed only transiently, e.g. an enzyme and substrate complex, a growth factor and its receptor interaction, or transcription factors assembled on a promoter.  To determine structures of macromolecular complexes, whether stable or transient, has become a common practice of structural biologists in the 21st century. (Full Article)


Click for Introduction and tips and tricks in Pre-crystallization modification, Crystallization, Post-crystallization treatment, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

Item 3: Topic Discussion

Click for previous discussions on: Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31/2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"
This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).