Newsletter 135
November 20, 2006

The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Advances in Protein Crystallography 24 - 25 January 2007, South San Francisco, CA, USA

9th International Conference on Biology and Synchrotron Radiation 13-17 August 2007, Manchester, England

Item 1: October 2006 Publications by Members:

 1:  Kar S, Choi EJ, Guo F, Dimitriadis EK, Kotova SL. 
 Right-handed DNA supercoiling by an octameric form of histone-like protein HU:
modulation of cellular transcription.
J Biol Chem. 2006 Oct 23; PMID: 17062578 
  
2:  Laronde-Leblanc N, Santhanam AN, Baker AR, Wlodawer A, Colburn NH. 
 Structural basis for inhibition of translation by the tumor suppressor Pdcd4.
Mol Cell Biol. 2006 Oct 23; PMID: 17060447 
  
3:  Jedrzejczak R, Dauter M, Dauter Z, Olszewski M, Dlugolecka A, Kur J. 
 Structure of the single-stranded DNA-binding protein SSB from Thermus
aquaticus.
Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1407-12.PMID: 17057346
  
4:  Schubot FD, Tropea JE, Waugh DS. 
 Structure of the POZ domain of human LRF, a master regulator of oncogenesis.
Biochem Biophys Res Commun. 2006 Dec 8;351(1):1-6. PMID: 17052694
  
5:  Tang C, Iwahara J, Clore GM. 
 Visualization of transient encounter complexes in protein-protein association.
Nature. 2006 Oct 15; PMID: 17051159
  
6:  Iwahara J, Clore GM. 
 Sensitivity improvement for correlations involving arginine side-chain
Nepsilon/Hepsilon resonances in multi-dimensional NMR experiments using
broadband (15)N 180 degrees pulses.
J Biomol NMR. 2006 Oct 12; PMID: 17036160
  
7:  Hu K, Vogeli B, Clore GM. 
 (13)C-detected HN(CA)C and HMCMC experiments using a single methyl-reprotonated
sample for unambiguous methyl resonance assignment.
J Biomol NMR. 2006 Oct 12; PMID: 17036159

Item 2: Tips and Tricks

Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

Item 3: Topic Discussion

Click for previous discussions on: Low Resolution Crystallography, PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

"Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage

with possible use of it for phasing"

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