Newsletter 133
October 23, 2006

The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Advances in Protein Crystallography 24 - 25 January 2007, South San Francisco, CA, USA

9th International Conference on Biology and Synchrotron Radiation 13-17 August 2007, Manchester, England

Item 1: September 2006 Publications by Members:

1:  Gustchina E, Louis JM, Bewley CA, Clore GM. 
 Synergistic Inhibition of HIV-1 Envelope-Mediated Membrane Fusion by Inhibitors
Targeting the N and C-Terminal Heptad Repeats of gp41.
J Mol Biol. 2006 Sep 12; PMID: 17010381 
   
2:  Iwahara J, Zweckstetter M, Clore GM. 
 NMR structural and kinetic characterization of a homeodomain diffusing and
hopping on nonspecific DNA.
Proc Natl Acad Sci U S A. 2006 Sep 28; PMID: 17008406
   
3:  Tang C, Clore GM. 
 A simple and reliable approach to docking protein-protein complexes from very
sparse NOE-derived intermolecular distance restraints.
J Biomol NMR. 2006 Sep;36(1):37-44. Epub 2006 Sep 12. PMID: 16967193
   
4:  Cheong CG, Hall TM. 
 Engineering RNA sequence specificity of Pumilio repeats.
Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13635-9. Epub 2006 Sep 5. 
PMID: 16954190

Item 2: Tips and Tricks - Crystallization

Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

Item 3: Topic Discussion - Low Resolution Crystallography

Byron DeLaBarre & Axel Brunger: Considerations for the refinement of low-resolution crystal structures

James Hurley (NIDDK): Low Resolution Crystallography in the Hurley Lab

Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

2005)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

"Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage

with possible use of it for phasing"

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).