Newsletter 118
March 27, 2006


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

The 36th Mid-Atlantic Macromolecular Crystallography Meeting

 

The 2006 Meeting of the American Crystallographic Association

 

Item 1: February 2006 Publications by Members:

1:  Li YF, Poole S, Rasulova F, Esser L, Savarino SJ, Xia D.
 Crystallization and preliminary X-ray diffraction analysis of CfaE, the
adhesive subunit of the CFA/I fimbriae from human enterotoxigenic Escherichia coli.
Acta Crystallograph Sect F. 2006 Feb 1;62(Pt 2):121-4. PMID: 16511280

2:  Prasanna MD, Vondrasek J, Wlodawer A, Rodriguez H, Bhat TN.
 Chemical compound navigator: A web-based chem-BLAST, chemical taxonomy-based search engine for browsing compounds.
Proteins. 2006 Feb 28; PMID: 16508960

3:  Natarajan K, Hicks A, Mans J, Robinson H, Guan R, Mariuzza RA, Margulies DH.
 Crystal Structure of the Murine Cytomegalovirus MHC-I Homolog m144.
J Mol Biol. 2006 Feb 9; PMID: 16500675

4:  Douek DC, Kwong PD, Nabel GJ.
 The rational design of an AIDS vaccine.
Cell. 2006 Feb 24;124(4):677-81. PMID: 16497577

5:  Ramakrishnan B, Ramasamy V, Qasba PK.
 Structural Snapshots of beta-1,4-Galactosyltransferase-I Along the Kinetic Pathway.
J Mol Biol. 2006 Feb 9; PMID: 16497331

6:  Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD.
 Crystal structure of group a streptococcus mac-1: insight into dimer-mediated specificity for recognition of human IgG.
Structure. 2006 Feb;14(2):225-35. PMID: 16472742

7:  Hurley JH, Emr SD.
 The ESCRT Complexes: Structure and Mechanism of a Membrane-Trafficking Network.
Annu Rev Biophys Biomol Struct. 2006 Feb 7; PMID: 16464152

8:  Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH.
 Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5.
Nat Struct Mol Biol. 2006 Mar;13(3):264-71. PMID: 16462746

9:  Yang W, Van Duyne GD.
 Protein-nucleic acid interactions: from A(rgonaute) to X(PF).
Curr Opin Struct Biol. 2006 Feb;16(1):1-4. PMID: 16439109

10:  Jaskolski M, Li M, Laco G, Gustchina A, Wlodawer A.
 Molecular replacement with pseudosymmetry and model dissimilarity: a case study.
Acta Crystallogr D. 2006 Feb;62(Pt 2):208-15. PMID: 16421452

11:  Jedrzejczak R, Dauter Z, Dauter M, Piatek R, Zalewska B, Mroz M, Bury K, Nowicki B, Kur J.
 Structure of DraD invasin from uropathogenic Escherichia coli: a dimer with swapped beta-tails.
Acta Crystallogr D. 2006 Feb;62(Pt 2):157-64. PMID: 16421447

12:  Ambudkar SV, Kim IW, Xia D, Sauna ZE.
 The A-loop, a novel conserved aromatic acid subdomain upstream of the Walker A motif in ABC transporters, is critical for ATP binding.
FEBS Lett. 2006 Feb 13;580(4):1049-55. PMID: 16412422

13:  Clore GM, Schwieters CD.
 Concordance of residual dipolar couplings, backbone order parameters and crystallographic B-factors for a small alpha/beta protein: a unified picture of high probability, fast atomic motions in proteins.
J Mol Biol. 2006 Feb 3;355(5):879-86. PMID: 16343537

14:  Saavedra JE, Srinivasan A, Buzard GS, Davies KM, Waterhouse DJ, Inami K, Wilde TC, Citro ML, Cuellar M, Deschamps JR, Parrish D, Shami PJ, Findlay VJ, Townsend DM, Tew KD, Singh S, Jia L, Ji X, Keefer LK.
 PABA/NO as an anticancer lead: analogue synthesis, structure revision, solution chemistry, reactivity toward glutathione, and in vitro activity.
J Med Chem. 2006 Feb 9;49(3):1157-64. PMID: 16451080


Item 2: Tips and Tricks

Sequence-to-Model in the Fast Lane: On any Swiss-Prot entry display, a click on the button "Submit a homology modeling request to SWISS-MODEL" will result in sequence comparison information and a homologous model for the entry. The button is the last of five located at the very bottom of the Swiss-Prot display.

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 3: Topic Discussion - Radiation Damage

Contributions on theoretical and practical aspects, and potential applications of radiation damage are appreciated.

Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Lectures

Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"

 

 

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).