Newsletter 114
January 30, 2006


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

The 36th Mid-Atlantic Macromolecular Crystallography Meeting

 

The 2006 Meeting of the American Crystallographic Association

 

Item 1: December 2005 Publications by Members:

1:  Townsend DM,  Findlay VJ,  Fazilev F, Ogle M, Fraser J, Saavedra J, Ji X, Keefer L, Tew KD.
 A Glutathione S-Transferase {pi} Activated Pro-drug Causes Kinase Activation Concurrent with S-glutathionylation of Proteins.
Mol Pharmacol. 2005 Dec 1; PMID: 16288082

1:  Hosseinpour F, Moore R, Negishi M, Sueyoshi T.
 Serine-202 regulates the nuclear translocation of constitutive
active/androstane receptor CAR.
Mol Pharmacol. 2005 Dec 23; PMID: 16377764

2:  Dauter Z.
 Current state and prospects of macromolecular crystallography.
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):1-11. Epub 2005 Dec 14. PMID: 16369088

3:  Li M, Laco GS, Jaskolski M, Rozycki J, Alexandratos J, Wlodawer A, Gustchina A.
 Crystal structure of human T cell leukemia virus protease, a novel target for anticancer drug design.
Proc Natl Acad Sci U S A. 2005;102(51):18332-7. PMID: 16352712

4:  Clore GM, Schwieters CD.
 Concordance of Residual Dipolar Couplings, Backbone Order Parameters and Crystallographic B-factors for a Small alpha/beta Protein: A Unified Picture of High Probability, Fast Atomic Motions in Proteins.
J Mol Biol. 2005 Dec 9; PMID: 16343537

5:  Cho S, Swaminathan CP, Yang J, Kerzic MC, Guan R, Kieke MC, Kranz DM, Mariuzza RA, Sundberg EJ.
 Structural basis of affinity maturation and intramolecular cooperativity in a
protein-protein interaction.
Structure (Camb). 2005 Dec;13(12):1775-87. PMID: 16338406

6:  Saxena AK, Singh K, Su HP, Klein MM, Stowers AW, Saul AJ, Long CA, Garboczi DN.
 The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms.
Nat Struct Mol Biol. 2005 Dec 4; PMID: 16327807

7:  Nallamsetty S, Austin BP, Penrose KJ, Waugh DS.
 Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.
Protein Sci. 2005 Dec;14(12):2964-71. PMID: 16322578

8:  Buchanan SK.
 Bacterial metal detectors.
Mol Microbiol. 2005 Dec;58(5):1205-9. PMID: 16313609

9:  Koike C, Moore R, Negishi M.
 Localization of the nuclear receptor CAR at the cell membrane of mouse liver.
FEBS Lett. 2005 Dec 19;579(30):6733-6. PMID: 16310787


10:  Laronde-Leblanc N, Wlodawer A.
 The RIO kinases: An atypical protein kinase family required for ribosome
biogenesis and cell cycle progression.
Biochim Biophys Acta. 2005 Dec 30;1754(1-2):14-24. PMID: 16182620

Item 2: Tips and Tricks

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 3: Topic Discussion

Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Lectures

Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"

 

 

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).