Newsletter 105
September 26, 2005


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Item 1: August 2005 Publications by Members:
 

1:  Burgess BR, Schuck P, Garboczi DN.
Dissection of merozoite surface protein 3, a representative of a family of
plasmodium falciparum surface proteins, reveals an oligomeric and highly
elongated molecule.
J Biol Chem. 2005 Aug 31; PMID: 16135515

2:  Ramagopal UA, Dauter Z, Thirumuruhan R, Fedorov E, Almo SC.
Radiation-induced site-specific damage of mercury derivatives: phasing and implications.
Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1289-98. Epub 2005 Aug 16. PMID: 16131763

3:  Vaisman A, Ling H, Woodgate R, Yang W.
Fidelity of Dpo4: effect of metal ions, nucleotide selection and
pyrophosphorolysis.
EMBO J. 2005 Sep 7;24(17):2957-67. PMID: 16107880

4:  Wlodawer A.
Giving credit where credit is due.
Nat Struct Mol Biol. 2005 Aug;12(8):634. PMID: 16077721

5:  Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ.
Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided
endoribonuclease, provides insights into RISC-mediated mRNA cleavage.
Mol Cell. 2005 Aug 5;19(3):405-19. PMID: 16061186

6:  Bell JK, Botos I, Hall PR, Askins J, Shiloach J, Segal DM, Davies DR.
The molecular structure of the Toll-like receptor 3 ligand-binding domain.
Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10976-80. Epub 2005 Jul 25. PMID: 16043704

7:  Hickman AB, Perez ZN, Zhou L, Musingarimi P, Ghirlando R, Hinshaw JE, Craig NL, Dyda F.
Molecular architecture of a eukaryotic DNA transposase.
Nat Struct Mol Biol. 2005 Aug;12(8):715-21. PMID: 16041385

8:  Pancera M, Lebowitz J, Schon A, Zhu P, Freire E, Kwong PD, Roux KH, Sodroski J, Wyatt R.
Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis.
J Virol. 2005 Aug;79(15):9954-69. PMID: 16014956

9:  Botos I, Melnikov EE, Cherry S, Kozlov S, Makhovskaya OV, Tropea JE, Gustchina A, Rotanova TV, Wlodawer A.
Atomic-resolution crystal structure of the proteolytic domain of Archaeoglobus fulgidus lon reveals the conformational variability in the active sites of lon proteases.
J Mol Biol. 2005 Aug 5;351(1):144-57. PMID: 16002085

10:  Williams DC Jr, Lee JY, Cai M, Bewley CA, Clore GM.
Crystal structures of the HIV-1 inhibitory cyanobacterial protein MVL free and bound to Man3GlcNAc2: structural basis for specificity and high-affinity binding to the core pentasaccharide from n-linked oligomannoside.
J Biol Chem. 2005 Aug 12;280(32):29269-76. PMID: 15937331


Item 2: Tips and Tricks

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 3: Topic Discussion

PHASER: MR with Maximum Likelihood

Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Lectures

Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"

 


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