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sk01-1l4y-fig2

Overall structure and representative electron density from MAD phasing of the MtSK•MgADP complex. MtSK contains eight alpha helices (blue ribbons), five beta strands (yellow arrows), and two 310 helices (blue ribbons) in three domains: LID, CORE, and SB. The ADP and HEPES molecules are shown as ball-and-stick models with atomic color scheme (C, black; N, blue; O, red; S, yellow; and P, purple) and the Mg2+ and Pt2+ ions are shown as bigger purple spheres. The initial electron density from MAD phasing is illustrated as green nets and contoured at the level of 1.0 sigma for Mg2+, Pt2+, ADP, HEPES, and Arg117 (Gu, Reshetnikova, Li, Wu, Yan, Singh, and Ji, J. Mol. Biol. 2002; 319:779-789. Abstract).