DNA Sequence

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DNA Sequence

Upload DNA sequence file (formats)

Parameters

Oligo Length: (format) (>20 nt)

Annealing Temperature: (format) (58-75*C)

Na+/K+ Concentration: 10 mM 25 mM 50 mM 75 mM 100 mM 150 mM 200 mM 250 mM 500 mM 750 mM 1 M

Mg2+ Concentration: 0 mM 0.5 mM 1.0 mM 1.5 mM 2 mM 3 mM 4 mM 5 mM 10 mM 20 mM 50 mM 100 mM 200 mM

Oligonucleotide Concentration: (1-100000 nM)

Thermodynamically Balanced Inside-Out Mode Output:

E-mail Address (optional)

E-mail Address:

Entering your e-mail address is entirely voluntary. The results will be made available online at the end of the program run, but if an address is entered, the results will also be sent to that address.